CD25 anticorps (FITC)

N° du produit ABIN6655112

N° du produit (Fournisseur): 200-302-n25

Cet anticorps anti-CD25 est un anticorps Souris Monoclonal détectant CD25 dans FACS et IHC. Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour CD25 anticorps (FITC) (ABIN6655112)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD25 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: BC96

Détail du produit anti-CD25 anticorps (FITC)

N° du produit (Fournisseur): 200-302-n25

Fournisseur: Rockland

Fonction: CD25 Fluorescein Antibody

Réactivité croisée (Details): Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque.

Purification: Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25.

Stérilité: Sterile filtered

Immunogène: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 4-6

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Commentaires:

Anti-CD25 is tested for Flow Cytometry and is useful for Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Date de péremption: 6 months

Références pour anticorps anti-CD25 (FITC) (ABIN6655112)

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." dans: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Waldmann: "The interleukin-2 receptor." dans: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, (1991) (PubMed).

Détails sur CD25

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25

Sujet:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55

Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Gene Name: IL2RA

ID gène: 3559

NCBI Accession: NP_000408

UniProt: P01589

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation